AGR2 Antibody (N-term)

• Catalog Number: orb1788146
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Predicted Reactivity: Mouse, Zebrafish
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: 13-42 aa
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 19979 Da
• Target: This Anterior Gradient 2 (AGR2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human Anterior Gradient 2 (AGR2).
• UniProt ID: O95994
• NCBI: NP_006399.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: AGR2; AG2; Anterior gradient protein 2 homolog; HP
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AGR2 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-AGR2 Antibody (N-term) at 1/1000 dilution. Lane 1: HT-29 whole cell lysates. Lane 2: T47D whole cell lysates. Lane 3: MCF-7 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 19 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent confocal image of A549 cell stained with AGR2 Antibody (N-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AGR2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). AGR2 immunoreactivity is localized to Cytoplasm significantly.