Acidic Cytokeratin Antibody (LMW / Type I)

• Catalog Number: orb1825131
• Category: Antibodies
• Description: This mAb recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 5048, 46, 45, and 40kDa.
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Human epidermal keratin was used as the immunogen for the Acidic Cytokeratin antibody.
• UniProt ID: Q7Z794
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Antibody Type: Primary Antibody
• Clone Number: rKRTL/8751
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only

IHC staining of FFPE human placental tissue with Acidic Cytokeratin antibody (clone rKRTL/8751). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Acidic Cytokeratin antibody (clone rKRTL/8751) as confirmation of integrity and purity.