Acetyl Lysine Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1974638
• Category: Antibodies
• Description: Acetyl Lysine Recombinant Rabbit Monoclonal Antibody
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: All
• Predicted Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: PBS, Glycerol, rAlbumin.
• Purification: Affinity purified by Protein A
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Clone Number: 6E11
• CAS Number: 56-87-1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Acetylated Lysine; Acetyllysine; Ac-lysine; Ac lys
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunoprecipitation of MCF-7 cell extract, IP antibody incubation conditions: orb1974638, 4°C overnight, 1:50 dilution, Incubation conditions for WB first antibody: H3K14ac, room temperature for 2 hours, 1:2000 dilution, Secondary antibody: Goat Anti Rabbit IgG (HRP), Sealing solution and Concentration: 5% NFDM/TBST dilution solution and Concentration: 5% NFDM/TBST, Swim Lane 1: 5% Input, Lane 2: Immunoprecipitation using rabbit monoclonal IgG homotypic control, Lane 3: Immunoprecipitation with orb1974638, Actual molecular weight: 15 kDa, Exposure time: 30 seconds.

Tissue: human liver, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1974638.

Tissue: mouse brain, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1974638.

Tissue: rat liver, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1974638.