ABCE1 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1173011
• Category: Antibodies
• Description: ABCE1 Recombinant Rabbit Monoclonal Antibody
• Target: ABCE1
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG/Kappa
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: A synthesized peptide derived from human ABCE1 (574-599aa)
• Tested applications: FC, ICC, IF, IHC-Fr, IHC-P, IP, WB
• Dilution range: WB=1:500-2000, ICC/IF=1:50-200, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200, Flow-Cyt=1:50-100, IP=1:20-50
• Antibody Type: Primary Antibody
• Clone Number: A12F4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ATP-binding cassette sub-family F(GCN20) member 1;
• Research Area: ATPases, Anti-Viral Signaling, Immunology, Signal
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: room temperature 2h, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1:HeLa, 2: Panc-1, 3: Mouse liver, Protein loading quantity: 20 µg, Exposure time: 10 s, Predicted MW: 67 kDa, Observed MW: 67 kDa.

Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1173011.

Cell line: NIH-3T3, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1173011.