5T4 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1816795
• Category: Antibodies
• Description: 5T4 Recombinant Rabbit Monoclonal Antibody
• Target: Tpbg
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human TPBG/5T4
• UniProt ID: Q13641
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Clone Number: 23B1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: 5T4; 5T4 oncofetal antigen; 5T4 oncofetal trophobl
• Research Area: Cancer Research, Developmental Biology, Stem Cell
• Note: For research use only

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: MCF-7, 2: JAR, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 46 kDa, Observed MW: 70-80/85 kDa.

IP of MCF-7 cells extracts, IP ab incubation condition: orb1816795, 4°C, overnight, 1:100 dilution, WB primary ab incubation condition: orb1816795, room temperature 2h, 1:1000 dilution, Secondary ab: Anti-Rabbit IgG for IP (HRP), Blocking buffer and Concentration: 5% , NFDM/TBST, Diluting buffer and Concentration: 5% , NFDM/TBST, 1: 5% Input, 2: IP with orb1816795, 3: IP with Rabbit monoclonal IgG Isotype, Observed MW: 70-80/85 kDa, Exposure time: 120 s.

Tissue: Human placenta, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:2000, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1816795.