ZO1 antibody

• Catalog Number: orb556544
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ZO1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human ZO-1. The exact sequence is proprietary.
• Concentration: 1 mg/ml
• Dilution range: WB: 1:500-1:3000
• Form/Appearance: Liquid: 1XPBS, 1% rAlbumin, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Purity: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• MW: 195
• Target: ZO1
• UniProt ID: Q07157
• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Buffer/Preservatives: 0.01% Thimerosal. 1XPBS (pH7), 1% rAlbumin, 20% Glycerol
• Alternative names: tight junction protein 1 , ZO-1
• Note: For research use only
• Application notes: Positive control: HCT116 , 293T , A431 , HeLa
• Expiration Date: 12 months from date of receipt.

Western blot analysis of 293T whole cell lysate(Lane 1), A431 whole cell lysate(Lane 2), HeLa whole cell lysate(Lane 3) using ZO1 antibody

Western blot analysis of HCT116 cell using ZO1 antibody