YAP antibody

• Catalog Number: orb154318
• Category: Antibodies
• Description: Rabbit polyclonal antibody to YAP. The transcriptional coactivator Yes-associated protein (YAP) has been shown to interact with and to enhance p73-dependent apoptosis in response to DNA damage. It has been shown that YAP requires the promyelocytic leukemia gene (PML) and nuclear body localization to coactivate p73. YAP imparts selectivity to p73 by promoting the activation of a subset of p53 and/or p73 target promoters (1). Akt promotes YAP localization to the cytoplasm, resulting in loss from the nucleus where it functions as a coactivator of transcription factors including p73. p73-mediated induction of Bax expression following DNA damage requires YAP function and is attenuated by Akt phosphorylation of YAP. YAP overexpression increases, while YAP depletion decreases, p73-mediated apoptosis following DNA damage, in an Akt inhibitable manner
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein of human YAP1(NP_001123617.1).
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-100
• Conjugation: Unconjugated
• MW: 72kDa
• UniProt ID: P46937
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.01% thiomersal,50% glycerol,pH7.3.
• Alternative names: anti YAP antibody, anti YKI antibody, anti YAP2 an
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of HeLa cell line using YAP antibody

Immunohistochemical staining of human gastric tissue using YAP antibody (dilution of 1:100)