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Catalog Number | orb1937947 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB29081 |
Tested applications | FC, IF, IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 90038 Da |
Target | This WDR27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-256 amino acids from the N-terminal region of human WDR27. |
UniProt ID | A2RRH5 |
NCBI | NP_872358.4, NP_001189479.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | WD repeat-containing protein 27, WDR27 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Confocal immunofluorescent analysis of WDR27 Antibody (N-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
WDR27 Antibody (N-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
WDR27 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of WDR27 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
WDR27 Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35 ug/lane). This demonstrates the WDR27 antibody detected the WDR27 protein (arrow).