VPS35 antibody

• Catalog Number: orb18631
• Category: Antibodies
• Description: Goat polyclonal antibody to VPS35
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Bovine, Human, Mouse, Rat
• Concentration:

  1-3 μg/ml

• Dilution range: ELISA: 1:128000, WB: 1.0-3.0 μg/ml, IHC-P: 5-8 μg/ml
• Conjugation: Unconjugated
• MW: 91.7
• Target: VPS35 / MEM3
• Entrez: 55737
• Protein Sequence: SPESEGPIYEGLIL
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Alternative names: anti VPS35 antibody, anti MEM3 antibody, anti FLJ1
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Testis, shows positive staining in Leydig cells, and cytoplasmic staining (dot-like) of some epithelial cells of the epididymis and of the rete testis.WB: Approx 90kDa band observed in Human Brain and Testis lysates (predicted MW of 91kDa according to NP_060676.2). Recommended for use at 1-3 μg/ml.Experiment Notes: Immunoflurescence: Staining of VPS35 (red) in human melanoma cells 1205LU: Please see Scott KL et al.; Nature. 2009 Jun 25;459(7250):1085-90.; PMID: 19553991.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Human (A) Mouse (B) and Rat (C) Brain lysate using VPS35 antibody.

Immunohistochemical staining of Human Prostate using VPS35 antibody

Immunohistochemical staining of Human Prostate using VPS35 antibody

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells using VPS35 antibody.

Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells using VPS35 antibody.

Western blot analysis of HepG2 (A) and HEK293 (B) cell lysate using VPS35 antibody.