Vimentin antibody

• Catalog Number: orb44572
• Category: Antibodies
• Description: Mouse monoclonal antibody to Vimentin
• Clonality: Monoclonal
• Clone Number: VI-10
• Tested applications: ICC, IHC-P, IP, WB
• Reactivity: Gallus, Human, Mouse, Porcine, Rat
• Isotype: Mouse IgM
• Concentration: 1 mg/ml
• Dilution range: Immunocytochemistry: Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive control: 3T3 murine Swiss albino fibroblast cell line, RBL rat basophilic leukemia cell line. Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml, positive tissue: skin fibroblast.Western blotting: Recommended dilution: 1-2 μg/ml.
• Purity: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Conjugation: Unconjugated
• Target: Vimentin
• Entrez: 7431
• UniProt ID: P08670
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Alternative names: anti CTRCT30 antibody, anti Epididymis luminal pro
• Note: For research use only
• Application notes: Immunocytochemistry: Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive control: 3T3 murine Swiss albino fibroblast cell line, RBL rat basophilic leukemia cell line. Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml, positive tissue: skin fibroblast.Western blotting: Recommended dilution: 1-2 μg/ml.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of chicken postnatal erythrocytes using Vimentin antibody

Immunoprecipitation analysis of HeLa cell lysate using Vimentin antibody

Immunofluorescence analysis of RBL rat basophilic cell line using Vimentin antibody