Vimentin antibody

• Catalog Number: orb44570
• Category: Antibodies
• Description: Mouse monoclonal antibody to Vimentin
• Clonality: Monoclonal
• Clone Number: VI-01
• Tested applications: FC, ICC, IHC-P, WB
• Reactivity: Mammal
• Isotype: Mouse IgM
• Immunogen: Pellet of porcine brain cold stable proteins after depolymerization of microtubules.
• Concentration: 1 mg/ml
• Dilution range: Immunocytochemistry: Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive control: 3T3 murine Swiss albino fibroblast cell line, RBL rat basophilic leukemia cell line. Flow cytometry: Recommended dilution: 1-5 µg/ml. Intracellular staining. Western blotting: Recommended dilution: 1 µg/ml.
• Purity: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Conjugation: Unconjugated
• Target: Vimentin
• Entrez: 7431
• UniProt ID: P08670
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Alternative names: anti CTRCT30 antibody, anti Epididymis luminal pro
• Note: For research use only
• Application notes: Immunocytochemistry: Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive control: 3T3 murine Swiss albino fibroblast cell line, RBL rat basophilic leukemia cell line.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of LEP-19 human fibroblast cell line (Lane 1

3) and 3T3 mouse fibroblast cell line (Lane 2