Villin Antibody

• Catalog Number: orb386095
• Category: Antibodies
• Description: Recognizes a protein of ~93kDa which is identified as Villin (VIL1). It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, Villin is predominantly expressed in tumors of colorectal origin. Antibody to Villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: VIL1/1314
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Amino acids 179-311 from the human protein were used as the immunogen for this Villin antibody.
• Dilution range: Western blot: 1-2ug/ml for 60 min at RT,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Villin antibody is available for research use only.
• UniProt ID: P09327
• Storage: Store the Villin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titration of the Villin antibody may be required for optimal performance.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human rectum tissue using Villin antibody

Immunohistochemical staining of Human colon using Villin antibody

Western blot analysis of human A549(lane 1), HepG2(lane 2) and HCT-116(lane 3) cell lysate using Villin antibody