VCAM-1 Antibody

• Catalog Number: orb749840
• Category: Antibodies
• Description: Recognizes a protein of 110kDa, identified as CD106 (also known as vascular cell adhesion molecule-1 (VCAM-1), L1CAM and INCAM-100). It is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. L1CAM serves as a ligand for leukocyte integrin (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses. This mAb inhibits the binding of leukocytes to L1CAM on stimulated endothelial cells.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: Activated human umbilical vein endothelial cells (HUVEC) were used as the immunogen for the VCAM1 antibody.
• UniProt ID: P19320
• Tested applications: FACS, IF
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml
• Application notes: Optimal dilution of the VCAM1 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: B-K9
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Store the VCAM1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Hazard Information: This VCAM1 antibody is available for research use only.
• Note: For research use only

SDS-PAGE Analysis of Purified, BSA-Free VCAM1 Antibody (clone B-K9). Confirmation of Integrity and Purity of the Antibody.