USF1 Antibody

• Catalog Number: orb1270399
• Category: Antibodies
• Description: USF1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This USF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 174-201 amino acids from the Central region of human USF1.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For IHC-P starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 34 kDa
• Target: USF1
• UniProt ID: P22415
• NCBI: P22415
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Upstream stimulatory factor 1, Class B basic helix
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For IHC-P starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis in mouse spleen tissue lysates (35 ug/lane).

Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Fluorescent confocal image of SK-BR-3 cell stained with USF1 Antibody. SK-BR-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with USF1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).USF1 immunoreactivity is localized to nucleus significantly.

USF1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.