UBR2 Antibody

• Catalog Number: orb1151897
• Category: Antibodies
• Description: Mouse monoclonal antibody to UBR2
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-UBR2-1D12
• Tested applications: FACS, IF, IHC-P, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: Recombinant full-length human UBR2 protein was used as the immunogen for the UBR2 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This UBR2 antibody is available for research use only.
• UniProt ID: Q8IWV8
• Storage: Aliquot the UBR2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the UBR2 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human thyroid tissue with UBR2 antibody (clone PCRP-UBR2-1D12) at 2 ug/ml. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

FACS staining of PFA-fixed human HeLa cells with UBR2 antibody (blue, clone PCRP-UBR2-1D12) and isotype control (red).

SDS-PAGE analysis of purified, BSA-free UBR2 antibody (PCRP-UBR2-1D12) as confirmation of integrity and purity.

Immunofluorescent staining of PFA-fixed human HeLa cells using UBR2 antibody (green, clone PCRP-UBR2-1D12) and phalloidin (red).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using UBR2 antibody (clone PCRP-UBR2-1D12). These results demonstrate the foremost specificity of the PCRP-UBR2-1D12 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.