UBE2L3 Antibody (C-term)

• Catalog Number: orb1788032
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This UBE2L3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 123-153 amino acids from the C-terminal region of human UBE2L3.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Immunogen: 123-153 aa
• UniProt ID: P68036
• MW: 17862 Da
• Tested applications: IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:50~100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Ubiquitin-conjugating enzyme E2 L3, L-UBC, UbcH7,
• Note: For research use only
• NCBI: NP_001243285.1, NP_003338.1, NP_001243284.1

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

All lanes: Anti-UBE2L3 Antibody (C137) at 1:1000 dilution. Lane 1: mouse testis lysates. Lane 2: rat testis lysates. Lane 3: human testis lysates. Lane 4: U87-MG whole cell lysates. Lane 5: K562 whole cell lysates Lane 6: PC-12 whole cell lysates Lane 7: NIH/3T3 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 18 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.