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    TRPV5 Antibody

    Catalog Number: orb570361

    DispatchUsually dispatched within 5-10 working days
    $ 191.00
    Catalog Numberorb570361
    CategoryAntibodies
    DescriptionTRPV5 Antibody
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsELISA, FC, ICC, IHC, IHC-Fr, WB
    ReactivityHuman, Mouse, Rat
    IsotypeRabbit IgG
    ImmunogenE.coli-derived human TRPV5 recombinant protein (Position: Q14-Q74+H636-F729).
    ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
    Dilution rangeWestern blot, 0.25-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry, 0.5-1μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml
    Form/AppearanceLyophilized
    ConjugationUnconjugated
    MW83 kDa
    UniProt IDQ9NQA5
    StorageStore at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
    Alternative namesTransient receptor potential cation channel subfam
    Read more...
    NoteFor research use only
    Application notesTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
    Expiration Date12 months from date of receipt.
    TRPV5 Antibody

    IHC analysis of TRPV5 using anti-TRPV5 antibody (orb570361). TRPV5 was detected in paraffin-embedded section of human pancreatic cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TRPV5 Antibody (orb570361) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.

    TRPV5 Antibody

    IHC analysis of TRPV5 using anti-TRPV5 antibody (orb570361). TRPV5 was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TRPV5 Antibody (orb570361) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.

    TRPV5 Antibody

    IHC analysis of TRPV5 using anti-TRPV5 antibody (orb570361). TRPV5 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TRPV5 Antibody (orb570361) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.

    TRPV5 Antibody

    Flow Cytometry analysis of HepG2 cells using anti-TRPV5 antibody (orb570361). Overlay histogram showing HepG2 cells stained with orb570361 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRPV5 Antibody (orb570361, 1μg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

    TRPV5 Antibody

    Flow Cytometry analysis of U87 cells using anti-TRPV5 antibody (orb570361). Overlay histogram showing U87 cells stained with orb570361 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRPV5 Antibody (orb570361, 1μg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

    TRPV5 Antibody

    Western blot analysis of TRPV5 using anti-TRPV5 antibody (orb570361). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human HeLa whole cell lysates, Lane 4: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPV5 antigen affinity purified polyclonal antibody (Catalog # orb570361) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for TRPV5 at approximately 83KD. The expected band size for TRPV5 is at 83KD.

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      Polyclonal

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      100 μg, 200 μg
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      Rabbit

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      Animal, Bovine, Canine, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat

      Canine, Equine, Guinea pig, Human, Mouse, Porcine, Rat

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      Polyclonal

      Unconjugated

      100 μl
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      IF,  WB

      Human, Mouse, Rat

      Rabbit

      Polyclonal

      Unconjugated

      200 μl, 100 μl, 50 μl
    • TRPV5 antibody [orb525281]

      ELISA,  IHC

      Human

      Rabbit

      Polyclonal

      Unconjugated

      50 μl, 100 μl
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