TRPV5 Antibody

• Catalog Number: orb1246826
• Category: Antibodies
• Description: TRPV5 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-SHRGWEILRQNT
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 85kDa band observed in Human Pancreas lysates (calculated MW of 82.6kDa according to NP_062815.2). Recommended concentration: 1-3ug/ml.Immunocytochemsitry/Immunofluorescence: This antibody has been successfully used in ICC/IF for human retinal pigment epithelium as described in the following paper: Kennedy et al, Mol Vis. 2010 Apr 14;16:665-75; PMID: 20405023.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: TRPV5
• UniProt ID: Q9NQA5
• NCBI: NP_062815.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: TRPV5 , transient receptor potential cation channe
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 85kDa band observed in Human Pancreas lysates (calculated MW of 82.6kDa according to NP_062815.2). Recommended concentration: 1-3ug/ml.Immunocytochemsitry/Immunofluorescence: This antibody has been successfully used in ICC/IF for human retinal pigment epithelium as described in the following paper: Kennedy et al, Mol Vis. 2010 Apr 14;16:665-75; PMID: 20405023.
• Expiration Date: 12 months from date of receipt.

orb1246826 (1 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.