Tropomyosin (phospho-Tyr490) antibody

• Catalog Number: orb7119
• Category: Tools
• Description: Tropomyosin (phospho-Tyr490) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human TrkA around the phosphorylation site of Tyr496 (PQ(p-Y)F)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/Test, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 90 kDa
• Target: Phospho-TrkA (Tyr496) +TrkB(Tyr516)
• UniProt ID: P04629
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: TrkA (Phospho-Tyr496); TrkA (Phospho Tyr496); TrkA
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraffin-embedded rat brain tissue fixed with 4% paraformaldehyde.Antigen retrieval by boiling with citrate buffer.Blocking buffer is goat serum(37 degrees for 20 min.).TrkA(phospho-Tyr490)antibody at 1:200 dilution with overnight incubation at 4 degrees