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Catalog Number | orb19785 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to TOM1L1 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, FC, IF, WB |
Reactivity | Human |
Dilution range | ELISA: 1:64000, WB: 0.3-1 μg/ml |
Conjugation | Unconjugated |
MW | 53 |
Target | TOM1L1 / SRCASM |
Entrez | 10040 |
Protein Sequence | EQNKNQKEATNTTSE |
RRID | AB_10767500 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti TOM1L1 antibody, anti SRCASM antibody, anti t Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 48kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 53.0kDa according to NP_005477.1). Recommended concentration: 0.3-1 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
1 ug/ml staining of A431 lysate 1 (A) + peptide (B), A431 lysate 2 (C) + peptide (D), HEK293 (E) + peptide (F), and HEL (G) + peptide (H) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
1 µg/ml staining of Human Cerebellum lysate (35 µg protein in RIPA buffer). Detected by chemiluminecence.