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TOM1L1 / SRCASM

Catalog Number: orb19785

DispatchUsually dispatched within 5-10 working days
$ 500.00
Catalog Numberorb19785
CategoryAntibodies
DescriptionGoat polyclonal antibody to TOM1L1
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, FC, IF, WB
ReactivityHuman
Dilution rangeELISA: 1:64000, WB: 0.3-1 μg/ml
ConjugationUnconjugated
MW53
TargetTOM1L1 / SRCASM
Entrez10040
Protein SequenceEQNKNQKEATNTTSE
RRIDAB_10767500
StorageAliquot and store at -20°C. Minimize freezing and thawing.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesanti TOM1L1 antibody, anti SRCASM antibody, anti t
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NoteFor research use only
Application notesELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 48kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 53.0kDa according to NP_005477.1). Recommended concentration: 0.3-1 μg/ml.
Expiration Date12 months from date of receipt.
TOM1L1 / SRCASM

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

TOM1L1 / SRCASM

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

TOM1L1 / SRCASM

1 ug/ml staining of A431 lysate 1 (A) + peptide (B), A431 lysate 2 (C) + peptide (D), HEK293 (E) + peptide (F), and HEL (G) + peptide (H) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

TOM1L1 / SRCASM

1 µg/ml staining of Human Cerebellum lysate (35 µg protein in RIPA buffer). Detected by chemiluminecence.

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    Rabbit

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