TIMP3 Antibody

• Catalog Number: orb1262365
• Category: Antibodies
• Description: TIMP3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Bovine, Monkey, Rabbit, Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This TIMP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113-139 amino acids from the Central region of human TIMP3.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 24 kDa
• Target: TIMP3
• UniProt ID: P35625
• NCBI: P35625
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Metalloproteinase inhibitor 3, Protein MIG-5, Tiss
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of TIMP3 Antibody in HepG2 cell line lysates (35 ug/lane)

Western blot analysis of TIMP3 Antibody Pab pre-incubated without (lane 1) and with (lane 2) blocking peptide in mouse NIH-3T3 cell line lysate.

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with TIMP3 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.