Tim-4 Antibody [5G3], Rabbit IgG

• Catalog Number: orb1671766
• Category: Antibodies
• Description: Tim-4 Antibody [5G3], Rabbit IgG
• Clonality: Recombinant
• Clone Number: 5G3
• Tested applications: Blocking, ELISA, FC, IF, IHC, WB
• Reactivity: Mouse
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunising female Lewis rats (Harlan Sprague-Dawley) with Tim-4:Ig fusion protein in complete Freund's adjuvant.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: Timd4
• UniProt ID: Q6U7R4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: TIMD4, SMUCKLER, SMUCKLER, TIM4, Spleen, mucin-con
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed mouse splenocytes with anti-Tim-4 antibody 5G3. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes permewith 0.15% Triton and stained with the chimeric mouse IgG1 version of 5G3 (orb1671766) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671766- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody (0) followed by Alexa Fluor® 488 secondary antibody.