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TEV Protein

TEV Protein

Catalog Number: orb169412

DispatchUsually dispatched within 5-10 working days
$ 250.00
Catalog Numberorb169412
CategoryProteins
DescriptionTobacco Etch Virus Protease Recombinant protein. TEV protease is the common name for the 27 kDa catalytic domain of the Nuclear Inclusion a (NIa) protein encoded by the tobacco etch virus (TEV). Because its sequence specificity is far more stringent than that of factor Xa, thrombin, or enterokinase, TEV protease is a very useful reagent for cleaving fusion proteins. TEV protease recognizes a linear epitope of the general form E-Xaa-Xaa-Y -Xaa-Q-(G/S), with cleavage occurring between Q and G or Q and S. The most commonly used sequence is ENLYFQG.
Form/AppearanceSterile liquid formulation.
PurityGreater than 90.0% as determined by analysis by SDS-PAGE.
SourceEscherichia Coli
StorageStability: rTEV although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles
Buffer/PreservativesThe rTEV contains 25mM Tris, pH 8.0, 75mM NaCl, 5mM EDTA, 10mM GSH, 50% Glycerol.
Alternative namesrTEV, TEV, P1 protease.
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NoteFor research use only
Application notesUnit Definition: One unit is defined as the amount of enzyme needed to cleave 3 ug of fusion protein in 1 hour to 85 % completion at 30°C in a buffer containing 50 mM Tris-HCl, pH 8.0, 0.5 mM EDTA, and 1 mM DTT. Cleavage Conditions: A number of variables can be changed to optimize the cleavage of any specific protein. The amount of rTEV, the temperature of the incubation, and the time needed for cleavage may be examined. If the protein of interest is heat-labile, then 4°C incubations are recommended. Reactions at 4°C will require longer incubation times and/or more rTEV
Expiration Date6 months from date of receipt.
  • TEV-Protease [orb2568757]

    Unconjugated

    not determined

    33 kDa (runs at 25–40 kDa on SDS-PAGE)

    E.coli

    1 mg
  • TEV Protease Protein [orb1476250]

    500 μg, 50 μg, 10 μg, 1 mg
  • Mouse CCL24 Recombinant Protein [orb2704613]

    98%

    10.3 kDa

    Yeast

    100 μg, 25 μg, 5 μg, 1 mg, 500 μg
  • Tobacco Etch Virus Protease protein [orb90006]

    Unconjugated

    Greater than 90.0% as determined by RP-HPLC and analysis by SDS-PAGE

    300 IU, 1 KIU
  • RecombinantTEVProtease,His [orb1494770]

    > 95% by SDS-PAGE analyses.

    28.4 kDa, observed by reducing SDS-PAGE.

    Escherichia coli.

    1000 IU, 10, 000 IU