TEV Protein

SKU: orb169412

Description

Tobacco Etch Virus Protease Recombinant protein. TEV protease is the common name for the 27 kDa catalytic domain of the Nuclear Inclusion a (NIa) protein encoded by the tobacco etch virus (TEV). Because its sequence specificity is far more stringent than that of factor Xa, thrombin, or enterokinase, TEV protease is a very useful reagent for cleaving fusion proteins. TEV protease recognizes a linear epitope of the general form E-Xaa-Xaa-Y -Xaa-Q-(G/S), with cleavage occurring between Q and G or Q and S. The most commonly used sequence is ENLYFQG.

Images & Validation

• Application Notes:
  Unit Definition: One unit is defined as the amount of enzyme needed to cleave 3 ug of fusion protein in 1 hour to 85 % completion at 30°C in a buffer containing 50 mM Tris-HCl, pH 8.0, 0.5 mM EDTA, and 1 mM DTT. Cleavage Conditions: A number of variables can be changed to optimize the cleavage of any specific protein. The amount of rTEV, the temperature of the incubation, and the time needed for cleavage may be examined. If the protein of interest is heat-labile, then 4°C incubations are recommended. Reactions at 4°C will require longer incubation times and/or more rTEV

Key Properties

• Source: Escherichia Coli
• Purity: Greater than 90.0% as determined by analysis by SDS-PAGE.

Storage & Handling

• Storage: Stability: rTEV although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles
• Form/Appearance: Sterile liquid formulation.
• Buffer/Preservatives: The rTEV contains 25mM Tris, pH 8.0, 75mM NaCl, 5mM EDTA, 10mM GSH, 50% Glycerol.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rTEV, TEV, P1 protease.