TERT Antibody

• Catalog Number: orb1268554
• Category: Antibodies
• Description: TERT Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This TERT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1104-1132 amino acids from human TERT.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 127 kDa
• Target: TERT
• UniProt ID: O14746
• NCBI: O14746
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Telomerase reverse transcriptase, HEST2, Telomeras
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:2000 dilution Lane 1: Raji whole cell lysate Lane 2: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis in Jurkat cell line and human normal uterus tissue lysates (35 ug/lane). This demonstrdetected the TERT protein (arrow).

Formalin-fixed and paraffin-embedded human lymph with TERT Antibody (S1125), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Confocal immunofluorescent analysis of TERT Antibody (S1125) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.