TEC Antibody

• Catalog Number: orb1262976
• Category: Antibodies
• Description: TEC Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This TEC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-205 amino acids from the Central region of human TEC.
• Concentration: batch dependent
• Dilution range: For IF starting dilution is: 1:10~50For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 74 kDa
• Target: TEC
• UniProt ID: P42680
• NCBI: P42680
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Tyrosine-protein kinase Tec, TEC, PSCTK4
• Note: For research use only
• Application notes: For IF starting dilution is: 1:10~50For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Expiration Date: 12 months from date of receipt.

Fluorescent image of Hela cell stained with TEC Antibody.Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TEC primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml).TEC immunoreactivity is localized to Cytoplasm significantly.

Fluorescent image of Hela cell stained with TEC Antibody.Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TEC primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml).TEC immunoreactivity is localized to Cytoplasm significantly.

Western blot analysis in NCI-H292 cell line lysates (35 ug/lane).

Western blot analysis of anti-TEC Pab in mouse liver tissue lysate

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.