Tau antibody

• Catalog Number: orb315602
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Tau
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Porcine, Rat, Sheep
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human TAU. The exact sequence is proprietary.
• Dilution range: WB: 1-500:1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: MAPT
• Entrez: 17762, 4137
• UniProt ID: P10636, P19332, P10637
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-AI413597 antibody, anti- AW045860 antibody, a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of TAU expression in DLD (A), mouse brain (B), mouse testis (C), rat brain (D), rat testis (E) whole cell lysates. (Predicted band size: 78 kD; Observed band size: 50-80 kD)

Immunofluorescent analysis of TAU staining in LS8 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).