Streptavidin protein

• Catalog Number: orb82632
• Category: Proteins
• Description: Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a KD of ~1×10-15 M. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native streptavidin.
• Tested applications: FA, HPLC, SDS-PAGE
• Purity:

  > 98% pure by SDS-PAGE and HPLC analyses.

• Conjugation: Unconjugated
• MW: 7.6 kDa
• Target: Streptavidin
• Solubility (25°C):

  Solubility: Clearly soluble in ddH2O (OD_405nm < 0.1 at 10 mg/ml water)

• Protein Sequence: MAEAGITGTW YNQLGSTFIV TAGADGALTG TYESAVGNAE SRYVLTGRYD SAPATDGSGT ALGWTVAWKN NYRNAHSATT WSGQYVGGAE ARINTQWLLT SGTTEANAWK STLVGHDTFT KVKPSAAS
• Biological Activity: Fully biologically active when compared to standard. The ED50 was determined by a cell proliferation assay using FDC-P1 cells is < 2.0 ng/ml, corresponding to a specific activity of > 5x10⁵ units/mg.
• Endotoxins: Less than 0.1 EU/μg of rStreptavidin as determined by LAL method.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: Lyophilized in 10 mM potassium phosphate buffer pH 6.5.
• Note: For research use only
• Application notes: Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Streptavidin protein