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Catalog Number | orb19595 |
---|---|
Category | Antibodies |
Description | Goat polyclonal to STEAP4 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Canine, Human, Mouse, Rat |
Dilution range | ELISA: 1:4000, WB: 0.3-1 μg/ml |
Conjugation | Unconjugated |
MW | 52 |
Target | STEAP4 / Dudulin4 |
Entrez | 79689 |
Protein Sequence | CVDNTLTRIRQGWERN |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti STEAP family member 4 antibody, anti DKFZp666 Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx. 50-55kDa band observed in Human Adipose lysates (calculated MW of 52.0kDa according to NP_078912.2). In transfected HEK293 transiently expressing Dudulin4 a band of approx. 52kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb19595 (0.3ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing Dudulin4 (RC216917) and probed with orb19595 (mock transfection in first lane), tested by Origene.
IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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