SRI-37240

SKU: orb2900816

Description

SRI-37240 is a potent premature termination codons (PTCs) inhibitor. SRI-37240 suppresses CFTR nonsense mutations. SRI-37240 alters cellular translation termination at PTCs in HEK293T cells. SRI-37240 can also restore CFTR function in primary bronchial epithelial cells when combination with G418.

Key Properties

• CAS Number: 883956-47-6
• MW: 385.46
• Purity: >98% (HPLC)
• Formula: C₂₄H₂₃N₃O₂
• SMILES: O=C1C=2C=CC=CC2N(C=3N=C(C=4C=CC=CC4)N(C(=O)C13)C5CCCCC5)C
• Target: CFTR
• Solubility: In Vitro: DMSO : 11.36 mg/mL (29.47 mM; Ultrasonic (<60°C)

Bioactivity

• In Vitro:
  SRI-37240 (1, 3, 10 and 30 μM; 48 h) induces concentration-dependent increases in CFTR-dependent (Forskolin-stimulated and sensitive to the inhibitor CFTRInh-172) chloride conductance. SRI-37240 (10 μM; 72 h) significantly increases the amount of full-length, fully glycosylated form of CFTR protein, and the unprocessed, immature form of full-length CFTR protein in 16HBEge cells when co-treated with G418 (100 μM). SRI-37240 (10 μM; 24 h) alters cellular translation termination at PTCs in HEK293T cells, also increases global densities of ribosomes at normal stop codons without affecting densities of ribosomes in 3-UTRs. SRI-37240 (10 μM; 72 h) restores CFTR function in primary bronchial epithelial cells when combination with G418. Western blot analysis. Cell line: CFTR-G542X 16HBEg e. Concentration: 10 μM. Incubation time: 24 h. Result: Significantly increased the amount of Band C CFTR protein, which represents the full-length, fully glycosylated form of CFTR and Band B, which represents the unprocessed, immature form of full-length CFTR protein when combined with G418 (100 μM).

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

CFTR