| Category | Primary antibodies |
|---|---|
| Catalog Number | orb98440 |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Tested applications | IHC-Fr, IHC-P, WB |
| Reactivity | Canine, Feline, Gallus, Human, Monkey, Porcine |
| Clone ID | R4A |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| UniProt ID | P53814 |
| Application notes | R4A is suitable for immunoblotting and immunohistochemistry on frozen and paraffin-embedded tissues. For staining paraffin-embedded tissues pretreatment in 10 mM citRate buffer (pH 6.0) and heating for 3 times 5 minutes in a microwave is required. Optimal antibody dilution should be determined by titRation; recommended range is 1:100-1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100-1:1000 for immunoblotting appliCations. |
| Storage | The antibody may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product. |
| Description | Mouse monoclonal to Smoothelin |
| Expiration Date | 12 months from date of receipt. |
| Note | For research use only. |
| Source | R4A is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal extract of chicken gizzard. |
- 2 images
-
- 1 imageSmoothelin antibody [orb158429]
ELISA, ICC, IF, WB, IHC
Bovine, Canine, Mouse, Porcine, Primate, Rabbit, Rat
100 μl, 200 μl, 50 μl -
- Smoothelin antibody [orb1420369]
ICC, IF, IHC-Fr, IHC-P, WB
Bovine, Canine, Porcine, Rat
Rabbit
Polyclonal
Unconjugated
100 μl - 1 image
-
- Smoothelin antibody [orb302026]
ICC, IHC-Fr, IHC-P, WB
Canine, Feline, Gallus, Human, Monkey, Porcine
Mouse
Monoclonal
100 μg
