You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb764334 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Smad3 (phospho-Ser425) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Ser425. AA range:376-425 |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
MW | 58 |
Storage | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Alternative names | Anti-SMAD3 antibody, anti-MADH3 antibody, anti-Mot Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of Hela cell. 1, Smad3 (phospho Ser425) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). ATG5 mouse Monoclonal Antibody (3C7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).
Immunofluorescence analysis of rat-lung tissue. 1, Smad3 (phospho Ser425) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.
Immunofluorescence analysis of rat-lung tissue. 1, Smad3 (phospho Ser425) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.
Immunofluorescence analysis of mouse-kidney tissue. 1, Smad3 (phospho Ser425) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.
IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IHC-P, WB | |
Bovine, Equine, Gallus, Human, Porcine, Rabbit | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating