Smad3 (phospho-Ser213) antibody

• Catalog Number: orb769034
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Smad3 (phospho-Ser213)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Ser213. AA range:179-228
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• MW: 48
• Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-SMAD3 antibody, anti-MADH3 antibody, anti-Mot
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Smad3 (Phospho-Ser213) Antibody.

Western blot analysis of lysates from HT29 cells, using Smad3 (Phospho-Ser213) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human brain tumor. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).