SFRP1 Antibody

• Catalog Number: orb527039
• Category: Antibodies
• Description: SFRP1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human SFRP1 recombinant protein (A204-K314).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 35 kDa
• UniProt ID: Q8N474
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Secreted frizzled-related protein 1; FRP-1; sFRP-1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A549 cells using anti-SFRP1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of SFRP1 using anti-SFRP1 antibody.Lane 1:human A549 cell;2:human U2OS cell;3:human PC-3 cell;4:rat heart tissue;5:mouse heart tissue;6:mouse HEPA1-6 cell.

IF analysis of SFRP1 using anti-SFRP1 antibody. SFRP1 was detected in immunocytochemical section of U20S cells.

IHC analysis of SFRP1 using anti-SFRP1 antibody.SFRP1 was detected in paraffin-embedded section of human colon cancer tissue.

IHC analysis of SFRP1 using anti-SFRP1 antibody.SFRP1 was detected in paraffin-embedded section of human lung cancer tissue.

IHC analysis of SFRP1 using anti-SFRP1 antibody.SFRP1 was detected in paraffin-embedded section of mouse intestine tissue.

IHC analysis of SFRP1 using anti-SFRP1 antibody.SFRP1 was detected in paraffin-embedded section of mouse kidney tissue.

IHC analysis of SFRP1 using anti-SFRP1 antibody.SFRP1 was detected in paraffin-embedded section of rat kidney tissue.

IHC analysis of SFRP1 using anti-SFRP1 antibody.SFRP1 was detected in paraffin-embedded section of rat intestine tissue.