What Are Secondary Antibodies?

Secondary antibodies are an effective research tool. Correctly chosen, they enable researchers to detect, purify and target antigens by binding to specific primary antibodies within immunoassays. Biorbyt provide conjugated and unconjugated secondary antibodies, and expert guidance to obtain optimal, consistent results.


Choice of secondary antibody conjugate is not dictated by the primary antibody. Using secondary antibodies is more cost-effective than labelling/conjugating your primary; the unconjugated primaries will be more able to recognise the target antigen.

To make detection easier:

  • Secondary antibodies can be conjugated to molecules including fluorophores and enzymes, e.g. Horseradish peroxidase (HRP).
  • They will localise a higher amount of conjugate at the antigen.

How to Select Secondary Antibodies for Optimal Results

Our new search tool simplifies the process. Simply use the drop down menus, from left to right to refine your search.
  1. Select your target species
  2. Select your host species (Rabbit, goat etc.) The secondary must recognise the primary)
  3. Choose specificity (IgA, IgG, IgM etc.)
  4. Choose antibody type (Whole IgG, F(ab')2, Fab)
  5. Select conjugate (if required)
  6. Select clonality
Using conjugated secondary antibodies - Immunofluorescence and flow cytometry need fluorescent reporter labels. ELISA and Western Blot are suited to chemiluminescent, colorimetric and fluorescence systems.