SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [84C4a]

• Catalog Number: orb1274607
• Category: Antibodies
• Description: SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [84C4a]
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: 84C4a
• Tested applications: ELISA
• Reactivity: Virus
• Isotype: IgG1k
• Immunogen: SARS-CoV-2 nucleoprotein (NP) specific peptide
• Concentration: batch dependent
• Dilution range: Paired with 75G5a, used for specific, high-sensitivity detection of SARS-CoV-2 nucleocapsid protein (NP).
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: N
• NCBI: QHD43423
• Storage: SARS-CoV-2°COVID-19) Nucleoprotein Antibody [8°C4a] can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: SARS-CoV-2 (COVID-19) Nucleoprotein Antibody [P002
• Note: For research use only
• Application notes: Paired with 75G5a, used for specific, high-sensitivity detection of SARS-CoV-2 nucleocapsid protein (NP).
• Expiration Date: 12 months from date of receipt.

Microtiter wells were coated with SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) and SARS-CoV NP at 1 ug/mL. Purified rabbit monoclonal antibody was serially diluted 1:2 starting at 1 ug/mL, and shows very strong and specific binding to COVID-19 NP antigen, with no significant cross-reactivity to SARS-CoV NP antigen.

Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Nucleocapsid in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody (orb1274607, 5 ng/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Nucleocapsid protein was observed in the macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.

A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies. COVID-19 NP was serially diluted 1:2 starting at 25 ng/mL.

Western blot analysis of SARS-CoV2 and SARS-CoV nucleocapsid protein (50 ng) probed with 1 ug/mL CoV2NP rabbit monoclonal antibody.