SAG Antibody

• Catalog Number: orb1260620
• Category: Antibodies
• Description: SAG Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-405 of human SAG (NP_000532.2).
• Concentration: batch dependent
• Dilution range: WB: 1:1000 - 1:2000IHC: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 48kDa
• Target: SAG
• UniProt ID: P10523
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: RP47, S-AG, S-arrestin, 48 kDa protein, S-antigen;
• Note: For research use only
• Application notes: WB: 1:1000 - 1:2000IHC: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using SAG antibody (orb1260620) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded mouse retina using SAG antibody (orb1260620) at dilution of 1:200 (40x lens).