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Catalog Number | orb20195 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to SAFB2 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA |
Reactivity | Human |
Dilution range | ELISA: 1:32000, WB: 1 μg/ml |
Conjugation | Unconjugated |
MW | 107 |
Target | SAFB2 |
Entrez | 9667 |
Protein Sequence | GEHNQRLEEHQARA |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti SAFB2 antibody, anti scaffold attachment fact Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave bands at approx 75kDa and 60kDa in lysates of HeLa cell lines after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 107kDa according to NP_055464.1. The 75kDa band was successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported |
Expiration Date | 12 months from date of receipt. |
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