S100A9 Antibody

• Catalog Number: orb1247383
• Category: Antibodies
• Description: S100A9 Antibody
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-DTNADKQLSFEEF
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: S100A9
• UniProt ID: P06702
• NCBI: NP_002956.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: S100A9, S100 calcium binding protein A9, 60B8AG, C
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 16kDa band observed in Human Peripheral Mononucleocyte lysates (calculated MW of 13.2kDa according to NP_002956.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (McCormick et al,; PMID: 16216873). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Lung shows nuclear staining in select cells. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Liver. Recommended concentration: 2.5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247383 (0.5 ug/ml) staining of Human Peripheral Mononucleocytes lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247383 (2.5 ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247383 (2.5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.