RRAGD Antibody

• Catalog Number: orb1264378
• Category: Antibodies
• Description: RRAGD Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This RRAGD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-199 amino acids from the Central region of human RRAGD.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 46 kDa
• Target: RRAGD
• UniProt ID: Q9NQL2
• NCBI: Q9NQL2
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Ras-related GTP-binding protein D, Rag D, RagD, RR
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:1000 dilution + 293T/17 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of RRAGD Antibody in MDA-MB435 cell line lysates (35 ug/lane)

Western blot analysis of RRAGD Antibody in mouse cerebellum tissue lysates (35 ug/lane)

RRAGD Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.