RPS6 Antibody (N-term)

• Catalog Number: orb1788410
• Category: Antibodies
• Description: Purified Mouse Monoclonal Antibody (Mab)
• Target: This RPS6 antibody is generated from a mouse immunized with RPS6 recombinant protein.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Immunogen: 1-239 aa
• UniProt ID: P62753
• MW: 28681 Da
• Tested applications: FC, IF, IHC-P, WB
• Dilution range: IF: 1:25, IF: 1:25, WB: 1:1000, WB: 1:1000, IHC-P: 1:25, IHC-P: 1:25, IHC-P: 1:25
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: 40S ribosomal protein S6, Phosphoprotein NP33, RPS
• Note: For research use only

Immunohistochemical analysis of paraffin-embedded H. brain section using RPS6 Antibody (N-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded M. kidney section using RPS6 Antibody (N-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. kidney section using RPS6 Antibody (N-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from mouse NIH/3T3, Hela cell line, rat brain tissue lysate (from left to right), using RPS6 Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.

Fluorescent image of Hela cells stained with RPS6 Antibody (N-term). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Fluorescent image of Hela cells stained with RPS6 Antibody (N-term). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

All lanes: Anti-RPS6 Antibody (N-term) at 1:1000 dilution. Lane 1: Hela whole cell lysates. Lane 2: HepG2 whole cell lysates. Lane 3: K562 whole cell lysates. Lane 4: NIH/3T3 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 29 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.