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Product Overview
Product Name RPE65 antibody
Catalog Number orb19749
Species/HostGoat
ReactivityCanine, Human, Rat
ConjugationUnconjugated
Tested applicationsELISA, WB
Immunogen C-EVKKNARKAPQPE
Target RPE65
Alternative Names
Product Properties
Form/Appearance Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Note For research use only.
ClonalityPolyclonal
Purity Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Uniprot ID Q16518
NCBI NP_000320.1
Entrez 6121
Product Description

Goat polyclonal to RPE65

Application Notes
Dilution Range ELISA: 1:32000, WB: 0.5-1.5 μg/ml
Application Notes ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.

WB: Approx 60kDa band observed in Rat Retina lysates (calculated MW of 60.9kDa according to Human NP_000320.1 and 61.0kDa according to Rat NP_446014.1). Recommended concentration: 0.5-1.5 μg/ml.
Validation Images
orb19749 (0.5ug/ml) staining of Rat Retina lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb19749 (0.5ug/ml) staining of Rat Retina lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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