ROR Alpha antibody

• Catalog Number: orb500782
• Category: Antibodies
• Description: ROR Alpha antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse ROR alpha
• Concentration: 1mg/ml
• Dilution range: WB=1:500-1000, IHC-P=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 59 kDa
• Target: ROR alpha
• UniProt ID: P35398
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: Preservative: 15mM Sodium Azide, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4
• Alternative names: NR1F1; Nuclear receptor ROR-alpha; Nuclear recepto
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: A431(human) Cell Lysate at 40 ug Primary: Anti-ROR alpha (orb500782) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD

Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-ROR alpha (orb500782) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD

Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Ant

Sample: Jurkat (Human) Cell Lysate at 40 ug Primary: Anti-ROR alpha(orb500782)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59kD Observed band size: 59 kD