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Product Overview
Product Name Robo1 antibody
Catalog Number orb19340
Species/HostGoat
ReactivityMouse
Predicted Reactivity Canine, Rat
ConjugationUnconjugated
Tested applicationsIHC-P, P-ELISA, WB
Immunogen Peptide with sequence C-QKARPAKKQKHQ, from the internal region of the protein sequence according to NP_062286.2.
Target Robo1
Alternative Names
Product Properties
Form/Appearance Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
Note For research use only.
ClonalityPolyclonal
Purity Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
MW 176 kDa
Uniprot ID Q9Y6N7
NCBI NP_062286.2
Product Description

Goat polyclonal antibody to Robo1

Application Notes
Dilution Range P-ELISA: 1:128000, WB: 0.05-0.2 μg/ml, IHC-P: 5 μg/ml
Application Notes ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.

WB: Preliminary experiments gave bands at approx 80kDa and 40kDa in lysates of Mouse Brain and Rat Brain after 0.03 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 176kDa according to NP_062286.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Validation Images
Western blot analysis of Mouse Brain lysate using Robo1 antibody
Western blot analysis of Mouse Brain lysate using Robo1 antibody
Immunohistochemical staining of Mouse Testis using Robo1 antibody
Immunohistochemical staining of Mouse Testis using Robo1 antibody
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