Robo1 antibody

• Catalog Number: orb19340
• Category: Antibodies
• Description: Goat polyclonal antibody to Robo1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Canine, Mouse, Rat
• Dilution range: ELISA: 1:128000, WB: 0.05-0.2 μg/ml, IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 176
• Target: Robo1 / DUTT1 (mouse)
• Protein Sequence: QKARPAKKQKHQ
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti Robo1 antibody, anti roundabout homolog 1 (Dr
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary experiments gave bands at approx 80kDa and 40kDa in lysates of Mouse Brain and Rat Brain after 0.03 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 176kDa according to NP_062286.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Mouse Brain lysate using Robo1 antibody

Immunohistochemical staining of Mouse Testis using Robo1 antibody