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RFK Antibody
Catalog Number: orb1255655
| Catalog Number | orb1255655 |
|---|---|
| Category | Antibodies |
| Description | RFK Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | WB |
| Reactivity | Human, Mouse |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-155 of human RFK (NP_060809.3). |
| Concentration | batch dependent |
| Dilution range | WB: 1:500 - 1:2000 |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | Observed: 14kDa |
| Target | RFK |
| UniProt ID | Q969G6 |
| Storage | Store at -20°C. Avoid freeze / thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Alternative names | RIFK, riboflavin kinase, 0610038L10Rik, ATP:ribofl Read more... |
| Note | For research use only |
| Application notes | WB: 1:500 - 1:2000 |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using RFK antibody (orb1255655) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
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