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Catalog Number | orb783355 |
---|---|
Category | Antibodies |
Description | REG3G Mouse Monoclonal Antibody |
Target | REG3G |
Clonality | Monoclonal |
Species/Host | Mouse |
Isotype | IgG1,k |
Conjugation | Unconjugated |
Reactivity | Human |
Form/Appearance | Liquid |
Concentration | 1mg/ml |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Immunogen | Recombinant human REG3G. |
UniProt ID | Q6UW15 |
MW | 17 kDa |
Tested applications | IF, IHC-Fr, IHC-P, WB |
Dilution range | WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:25, IF=1:100-500 |
Antibody Type | Primary Antibody |
Clone Number | F6D9 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Reg III gamma; REG3G_HUMAN; Reg3 gamma; Reg3gamma; Read more... |
Note | For research use only |
Paraformaldehyde-fixed, paraffin embedded (human pancreas tissue sections), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (REG3G) Monoclonal Antibody, Unconjugated (orb783355) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Sample: Lane 1: Human Pancreas tissue lysates, Primary: Anti-REG3G (orb783355) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 17 kD, Observed band size: 17 kD.