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Catalog Number | orb1714480 |
---|---|
Category | Proteins |
Description | Recombinant Human Osteopontin |
Tested applications | ELISA, WB |
Reactivity | Human |
Tag | C-terminal His Tag |
Purity | Greater than 90% as determined by SDS-PAGE |
MW | 60-65 kDa |
UniProt ID | P10451 |
Source | HEK293 cells |
Expression Region | 17-314 |
Storage | -20°C for 12 months as lyophilized; 2-8°C for 1 month under sterile conditions after reconstitution |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution in PBS with 5% trehalose, pH 7.4 |
Alternative names | BNSP, Bone sialoprotein 1, BSPI, ETA 1, Nephropont Read more... |
Note | For research use only |
Application notes | Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/ml in sterile distilled water by gentle pipetting 2-3 times, don’t vortex |
Expiration Date | 6 months from date of receipt. |
> 95 % by SDS-PAGE analyses. | |
Apparent molecular mass of 60-65 kDa in SDS-PAGE under reducing conditions, a single glycosylated polypeptide protein consisting of 290 amino acids including a C-terminal 6x polyhistidine tag. | |
Mouse myeloma cell line, NS0 |
Unconjugated | |
90% | |
33.0 kDa | |
Human Osteopontin, His Tag (orb257724) is expressed from human 293 cells (HEK293). It contains AA Ile 17 - Asn 300 (Accession # AAH07016). |
Greater than 85% as determined by SDS-PAGE. | |
62.2 kDa | |
Mammalian cell |
Greater than 90% as determined by SDS-PAGE. | |
34.2 kDa | |
Yeast |
> 90% as determined by SDS-PAGE | |
34.8 kDa |