Rat TNF alpha protein

• Catalog Number: orb80110
• Category: Proteins
• Description: Rat TNF alpha protein
• Tested applications: FA, HPLC, SDS-PAGE
• Purity: > 97.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Conjugation: Unconjugated
• Target: Rat TNF alpha
• Solubility (25°C): It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Arg-Ser-Ser.
• Biological Activity: The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin is 0.05ng/ml, corresponding to Specific Activity of 20,000,000 IU/mg.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: The concentrated protein solution (1mg/ml) was lyophilized from 20 mM phosphate buffer and 0.1M NaCl.
• Alternative names: APC1 protein, Cachectin protein, DIF protein, Diff
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Rat TNF alpha protein