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Catalog Number | orb82622 |
---|---|
Category | Proteins |
Description | Rat IL21 protein |
Target | Rat IL21 |
Conjugation | Unconjugated |
Buffer/Preservatives | Sterile Filtered White lyophilized (freeze-dried) powder. We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. |
Purity | > 95% pure by SDS-PAGE and HPLC analyses. |
Protein Sequence | HKSSPQRPDH LLIRLRHLM D IVEQLKIY EN DLDPELL TAP QDVKGQ CEHE AFACF QKAKL KPSN TGNNKT FIN DLLAQLR RR LPAKRTGN K QRHMAKCPS CDLYEKKTPK EFLERLKWL L QKMIHQHL S |
MW | 15.2 kDa |
Tested applications | FA, HPLC, SDS-PAGE |
Application notes | Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions. |
Biological Origin | E.coli |
Biological Activity | Measured by its ability to inhibit IL21dependent proliferation of N1186 human T cells. The ED50 for this effect is typically 1-5μg/mL in the presence of 50 ng/mL of recombinant mouse IL21. Less than 1EU/μg of rRtIL21 as determined by LAL method. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Alternative names | IL 21 protein, IL21 protein, IL-21 protein, Interl Read more... |
Note | For research use only |
SDS-PAGE analysis of Rat IL21 protein
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
16 kDa | |
E.Coli |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
35.8 kDa | |
E.Coli |
> 90%, determined by SDS-PAGE | |
This protein contains the rat IL21 (NP_001102413.1) (Met1-Ser146) was expressed with a polyhistide tag at the C-terminus and expressed from Baculovirus-Insect Cells. |