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Catalog Number | orb80069 |
---|---|
Category | Proteins |
Description | IL-10 Recombinant Rat produced in E.coli is a single, glycosylated polypeptide chain containing 160 amino acids and having a molecular mass of 18.7 kDa. The IL10 Mouse is purified by proprietary chromatographic techniques. |
Tested applications | FA, HPLC, SDS-PAGE |
Purity | > 95.0% as determined by RP-HPLC and analysis by SDS-PAGE |
Conjugation | Unconjugated |
Target | rIL 10 |
Solubility (25°C) | It is recommended to reconstitute the lyophilized IL-10 Rat Recombinant in sterile H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Lys-Gly-His. |
Biological Activity | The ED50 as determined by the dose dependent inhibition of MC/9 proliferation is less than 10 ng/ml. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | The protein was lyophilized containing 25mM sodium phosphate buffer pH-7.25 and 100mM NaCl. |
Alternative names | B-TCGF protein, CSIF protein, TGIF protein, IL-10 Read more... |
Note | For research use only |
Application notes | Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.62 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of IL-10 as Reference Standard. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
39.9 kDa | |
Rat TIGIT, Fc Tag (orb383521) is expressed from human 293 cells (HEK293). It contains AA Ala 17 - Ala 138 (Accession # D3ZTQ2). |
Unconjugated | |
90% | |
27.1 kDa | |
Rat B7-H4, His Tag (orb1152428) is expressed from human 293 cells (HEK293). It contains AA Phe 29 - Gly 257 (Accession # Q501W4-1). |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
17.5 kDa | |
E.Coli |
8-22 kDa, observed by reducing SDS-PAGE. | |
CHO |
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