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Catalog Number | orb80025 |
---|---|
Category | Proteins |
Description | Rat FGF basic protein |
Tested applications | FA, HPLC, SDS-PAGE |
Purity | > 97.0% as determined by RP-HPLC and analysis by SDS-PAGE |
Conjugation | Unconjugated |
Target | Rat FGF basic |
Solubility (25°C) | It is recommended to reconstitute the lyophilized Rat bFGF in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | PALPEDGGGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHV KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTEE CFFFERLESN NYNTYRSRKY SSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS. |
Biological Activity | The ED50 range as determined by the dose-dependant proliferation of BALB/3T3 cells was found to be less than 0.2 ng/ml, corresponding to specific activity of 5,000,000 IU/mg. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | FGF-b was lyophilized from mg/ml solution after extensive dialysis against 20 mM phosphate buffer, pH 7.4 and 130mM NaCl. |
Alternative names | HBGH-2 protein, HBGF-2 protein, Prostatropin prote Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Rat FGF basic protein
Greater than 95% as determined by SDS-PAGE. | |
16.2 kDa | |
E.coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
> 95% by SDS-PAGE. | |
KMP1783, Recombinant Rat FGF-2/FGF basic/bFGF Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ala11-Ser154) of rat FGF-2/FGF basic/bFGF (Accession #P13109). |
≥90% as determined by SDS-PAGE | |
This protein contains the rat FGF2(Ala11-Ser154) was fused without Tag and expressed in E. coli. |
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